Determination of mite Allergens in House Dust Using the Enzyme Immunoassay

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Determination of mite allergens in house dust using the enzyme immunoassay.

The aim of this study was to determine the level of two major mite allergens Dermatophagoides pteronyssinus (Der p 1) and Dermatophagoides farinae (Der f 1) in 30 urban homes in Zagreb, Croatia, using the enzyme immunoassay with two monoclonal antibodies which has been established as the reference method for indoor allergen analysis. Dust samples were taken by vacuuming a carpeted area and coll...

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House dust mite allergens in domestic homes in Cheonan, Korea.

House dust mites produce inhalant allergens of importance to allergic patients. We measured the major group 1 allergens, Der p 1 and Der f 1, from the house dust mites Dermatophagoides pteronyssinus and Dermatophagoides farina, respectively in 100 randomly selected domestic homes from Cheonan, Korea. Dust samples were collected by vacuuming from the living room floor and 1 mattress in each home...

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House dust mite allergens in asthma and allergy.

IgE antibodies in house dust mite (HDM) allergy follow a predictable pattern. Half are directed against two dominant allergens and the remainder largely against four midpotency allergens. This hierarchical pattern is not changed by the titre of the IgE response or severity of disease. The structures of these allergens are known and they can be produced as authentic recombinant allergens. There ...

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House dust mite allergy

The importance of the allergens in sensitisation and disease can be readily demonstrated in regions with and without mite infestation. Dose-responce relationships in regions with high infestation are less easy to detect pointing to the importance of variations of other factors. The relationship between allergic disease and IgE antibody shows that only people with very high responses become pers...

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Protective effect of the DNA vaccine encoding the major house dust mite allergens on allergic inflammation in the murine model of house dust mite allergy

BACKGROUND Vaccination with naked DNA encoding antigen induces cellular and humoral immunity characterized by the activation of specific Th1 cells. OBJECTIVE To evaluate the effects of vaccination with mixed naked DNA plasmids encoding Der p 1, Der p 2, Der p 3, Der f 1, Der f 2, and Der f 3, the major house dust mite allergens on the allergic inflammation to the whole house dust mites (HDM) ...

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ژورنال

عنوان ژورنال: Archives of Industrial Hygiene and Toxicology

سال: 2007

ISSN: 0004-1254

DOI: 10.2478/v10004-007-0034-2